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Invited Presentation

SPSJ

SPSJ02: Advancements in UV Raman Spectroscopy: Fundamental Studies and Practical Applications

Quantitative analysis of fatty chain composition in fat of live cell and tissue by Raman spectroscopy

Monday, October 6, 2025
2:50 PM - 3:10 PM EDT
Location: Meeting Room #6

    Non-Presenting Author(s)

  • PP

    Pradjna Paramitha

    School of Biological and Environmental Sciences, Kwansei Gakuin University
    Sanda, Hyogo, Japan

  • BA

    Bibin Andriana

    School of Biological and Environmental Sciences, Kwansei Gakuin University
    Sanda, Hyogo, Japan

  • KI

    Keita Iwasaki, PhD

    Postdoctoral Researcher
    School of Biological and Environmental Sciences, Kwansei Gakuin University
    Sanda, Hyogo, Japan

  • YO

    Yurika Otoki, Ph.D.

    Assistant Professor
    Graduate School of Agricultural Science, Tohoku University
    Sendai, Miyagi, Japan

  • IK

    Ibuki Kusumoto

    Graduate School of Agricultural Science, Tohoku University
    Sendai, Miyagi, Japan

  • KN

    Kiyotaka Nakagawa

    Graduate School of Agricultural Science, Tohoku University
    Sendai, Miyagi, Japan

    • Presenting Author(s)

  • HS

    Hidetoshi Sato

    Professor
    Kwansei Gakuin University
    Sanda, Hyogo, Japan

    • Submitter(s)

  • HS

    Hidetoshi Sato

    Professor
    Kwansei Gakuin University
    Sanda, Hyogo, Japan

In fifteen words or less, explain the significance of this contribution (Novel Aspect).:

A technique to build analytical models for TGs using modified spectra of FAs

Abstract Text:

The main component of fat in a body is triacylglycerol (TG). The TG molecule consists of three fatty acid (FA) chains and glycerol which are connected with ester bonding. A several types of FA are found in mammals, such as palmitic acid (PA, 16:0), stearic acid (SA, 18:0), oleic acid (OA, 18:1) linoleic acid (LA, 18:2) and so on. In our previous study, it was suggested that LA as well as saturated FAs, such as PA and SA, induced cell death of a liver model cell, HepG2 cell. The fact that some FAs could be toxic to specific cells, suggesting that the FA composition in body would give effects to the health of total body. The purpose of the present study is to develop techniques to estimate the quantitative compositions of the FAs in fat. We employ Raman spectroscopy and chemometrics to monitor the FAs compositions in live cells and tissues to know the metabolism of FA chains. To obtain a quantitative result with Raman spectroscopy, it is necessary to build a reliable analytical model by using many calibration samples. Since the combinations of the three FA chains are varied in the fat, it is impractical to prepare the pure samples of various TGs to build an analytical model. We employed methyl ester of FAs as calibration samples instead of the TGs. The spectra of calibration samples were modified with our new method based on PCA to mimic the spectra of pure TGs. Although the pure TGs samples are not available, we successfully obtained the analytical model of FAs in the fat.